应用可视光组织氧监测急性心肌缺血模型的舌诊研究

Tongue Diagnosis Study of Tissue Oxygen Monitoring of Acute Myocardial Ischemia Rat Model by Visible Light

目的观察急性心肌缺血大鼠模型的舌及心脏氧饱和度(stO2)变化以及对药物干预的反应。方法将健康SD雄性成年大鼠随机分为假手术组、模型组、西药组(美托洛尔0.2mg/kg)、大蒜素组(2.7mg/kg)、大蒜素普通脂质体组(2.7mg/kg)、大蒜素长循环脂质体组(2.7mg/kg)。制备急性心肌缺血再灌注大鼠模型,测验舌及心脏表面的stO2。结果在结扎前、给药后10min、再灌注5min时,心脏与舌比较,stO2下降更明显。从模型组能看出舌stO2在开胸手术后的各时间段与实验前比较都有明显下降。药物干预与舌stO2的影响不显著。除了假手术组以外,各组心脏stO2在结扎后和给药后,具有明显的下降,再灌注后则显著回升。结论急性心肌缺血可使舌stO2下降,但较心脏反应缓慢。本研究所选的药物对造模后stO2的影响不显著。

Objective： To observe the stO2 changes of tongue and heart of acute myocardial ischemia rat model and its reaction for drug intervention. Methods： The normal SD male rats were randomly divided into sham-operated group, model group, western medicine group （metoprolol 0. 2mg/kg）, garlicin group （2.7mg/kg）, garlicin conventional liposomes group （2. 7mg/kg）, garlicin long circulating liposome group （2. 7mg/kg）. The myocardial ischemia reperfusion rat model was established. The stO2 of its tongue and heart surface was examined. Results： At the pre-oeclusion, 10 rain after giving medicine, 5 min after reperfusion, the stO2 of heart was declined more than that of tongue remarkably. Compared with pre-operation, the stO2 of tongue was declined obviously in model group. Medical intervention had no obvious affect on tongue stO2. Except of sham-operated group, the heart stO2 was declined in comparison after ligation and given medicine, and it reascended in each group after reperfusion. Conclusion： The acute myocardial isehemia could decline the stO2 of tongue, but the reaction is slower than that of heart. The medicine selected in this study have no obvious influence on the stO2 after model establishment.