表面活性剂对两性霉素B溶解行为的影响

Solubilization Effect of Surfactant on Amphotericin B

目的:研究不同表面活性剂对两性霉素B溶解行为的影响。方法:选用非离子表面活性剂司盘-20、吐温-40、吐温-60、吐温-80、泊洛沙姆188,阳离子表面活性剂十六烷基三甲基氯化铵,阴离子表面活性剂十二烷基磺酸钠、脱氧胆酸钠及两性离子表面活性剂卵磷脂,配成系列浓度的表面活性剂溶液,通过紫外分光光度法,以最大增溶百分比为指标考察其对两性霉素B溶解度的影响。结果:在0.2～4mg·mL-1浓度范围内,各表面活性剂最大增溶百分比司盘-20为1365.44%(4mg·mL-1)、吐温-40为353.46%(0.8mg·mL-1)、吐温-60为1165.30%(4mg·mL-1)、吐温-80为973.46%(0.4mg·mL-1)、泊洛沙姆188为1527.95%(4mg·mL-1)、十二烷基磺酸钠为1199.16%(4mg·mL-1)、脱氧胆酸钠为88.62%(4mg·mL-1)、十六烷基三甲基氯化铵为1671.68%(4mg·mL-1)、卵磷脂为314.36%(4mg·mL-1)。结论:本试验中,表面活性剂司盘-20、吐温-60、吐温-80、泊洛沙姆188、十二烷基磺酸钠、脱氧胆酸钠、十六烷基三甲基氯化铵对两性霉素B具有显著的增溶效果;而吐温-40、脱氧胆酸钠、卵磷脂增溶效果不明显。

OBJECTIVE： To study the solubilization effect of different surfactant on amphotericin B. METHODS： Surfaceactive solution with series of concentrations were prepared by several agents including span-20, tween-40, tween-60, tween-80 and poloxamer 188 as non-ionic surfactant, hexadecyltrimethylammonium chloride （HTMAC） as cationic surfaceactive agent, sodium dodecyl benzene sulfonate （SDBS） and sodium deoxycholate as anionic surfaceactive agent, lecithin as bi-ionactiveagent. The solubilization effect of surfactant on amphotericin B was determined by UV spectrophotometry with maximum solubilization percentage as index. RESULTS： Maximum solubilization ratio in the range of 0.2-4.0 mg·mL^-1 were as follows： 1 365.44% for span-20 （4 mg·mL^-1）, 353.46% for tween-40 （0.8 mg·mL^-1）, 1 165.30% for tween-60 （4 mg·mL^-1）, 973.46% for tween-80 （0.4 mg·mL^-1）, 1 527.95% for poloxamer 188 （4 mg·mL^-1）, 1 199.16% for sodium dodecyl benzene sulfonat （4 mg·mL^-1）, 1 671.68% for hexadecyltrimethylammonium chloride （4 mg·mL^-1）, 88.62% for sodium deoxycholate （4 mg·mL^-1） and 314.36% for lecithin （4 mg·mL^-1）. CONCLUSIONS： Surfactant span-20, tween-60, tween-80, poloxamer 188, sodium dodecyl benzene sulfonat and hexadecyltrimethylammonium chloride have remarkable solubilization effect on amphotericin B. But tween-40, sodium deoxycholate and lecithin have unapparent solubilization effect on amphotericin B.