HPLC法测定硫普罗宁葡萄糖注射液中硫普罗宁的含量

Determination of Tiopronin in Tiopronin/Glucose Injection by HPLC

目的:建立高效液相色谱法测定硫普罗宁葡萄糖注射液中硫普罗宁的含量。方法:色谱柱采用Diamonsil-C18,流动相为0.07mol·L-1磷酸二氢钾溶液,流速为1.0mL·min-1,检测波长为210nm。结果:硫普罗宁检测浓度在24.17～56.39μg·mL-1范围内线性关系良好(r=0.9995);平均回收率为99.06%,RSD=0.7%。结论:本方法简便、快速、准确、专属性强,适用于硫普罗宁葡萄糖注射液中硫普罗宁的含量测定。

OBJECTIVE： To establish an HPLC method for the determination of tiopronin in Tiopronirdglucose injection. METHODS： The separation was performed on Diamonsil-C18 column with flow rate of 1 mL·min^-1. The mobile phase composed of 0.07 mol·L^-1 NaH2PO4. The detection wavelength was 210 nm. RESULTS： Tiopronin showed good linearity within range of 24.17-56.39 μg·mL^-1（r=0.999 6）. The average recovery was 99.06% （RSD=0.7%）. CONCLUSION： The method is simple, rapid, accurate and specific for the determination of tiopronin in Tiopronin/glucose injection.