摘要
目的探讨自身抗体检测对自身免疫性肝病诊断的意义。方法采用间接免疫荧光法检测抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA)和用免疫印迹法对抗可溶性肝抗原/肝胰抗原(抗SLA/LP)、抗肝肾微粒体抗体Ⅰ型(抗LKM-1)和抗肝特异性胞浆抗原Ⅰ型抗体(抗LC-1)等肝脏疾病相关的自身抗体进行检测。另用酶联免疫吸附实验检测AMA-M2。结果自身免疫性肝病中,自身免疫性肝炎(AIH)组SLA/LP阳性率20.0%,LKM-1阳性率16.0%,LC-1阳性率8.0%,SMA阳性率52.0%,AMA阳性率12.0%,AMA-M2阳性率4.0%;原发性胆汁性肝硬化(PBC)组SLA/LP阳性率2.6%,SMA阳性率15.4%,AMA阳性率94.9%,AMA-M2阳性率为97.4%。原发性硬化性胆管炎(PSC)和非自身免疫性肝病组抗肝抗原自身抗体阳性检出率为0,健康体检者出未检出抗肝抗原自身抗体。自身免疫性肝病组中AIH和PBC的阳性检出率明显高于非自身免疫性肝病组,差异有统计学意义(χ2=34.46,P<0.05)。结论自身抗体检测对自身免疫性肝病诊断、分型具有较高的临床价值。
Objective To analyzed clinical significance of dectection of autoantibodies in patients with autoimmune liver disease. Methods Indirect immunofluorescent assay was used to detect anti-nuclear antibodies （ANA）,antimitochondria antibodies （AMA）,and anti-smooth muscle antibodies （SMA）. Immune blotting assay was used to detect soluble liver antigen （SLA）/liver-pancreas antigen（LP）, anti-liver kidney mierotonal antibody-1 （LKM-1）, liver cytosolic-1 （.LC-1）. Enzyme-linked immune absorbent assay was used to measure subtype of AMA （M2）. Results The autoimmune hepatopathy （AIH） group could be divided into 3 subtypes according to the difference of the autoantibodies as Ⅰ （75.4%）, Ⅱ（10.3%）, Ⅲ （17.2%）. The positive rates of SLA/LP,LKM-1, LC-1 ,SMA,AMA and AMA-M2 were 20.0%, 16.0%, 8.0%, 52.0% ,12.0 and 4.0% respectively in patients with autoimmune hepatitis. The positive rate of SLA/LP,SMA,AMA and AMA-M2 were 2.6% ,15.4% ,94.9 and 97.4% in patients with primary biliary cirrhosis （PBC） respectively. While the positive reate was 1.20% in non-autoimmune hepatopathy group. No autoant/bodies associated with autoimmune liver disease were detected in PSC,non-autoimmune live diseases group and healthy group. Conclusions The AIH and PBC in autoimmune liver diseases are progressive liver inflammation accompanied with the production of characteristic antibodies. Detection of autoantibodies is clinically useful for identifying and classifying liver diseases.
出处
《中国热带医学》
CAS
2010年第2期159-161,共3页
China Tropical Medicine
