进展期胃癌血清VEGF-D的表达与其侵袭和淋巴道转移的关系

Expression and significance of serum vascular endothelial growth factor D in gastric carcinoma patients at advanced stage

目的研究进展期胃癌患者血清中血管内皮生长因子D(VEGF-D)的水平,并了解其与进展期胃癌的侵袭和淋巴道转移的关系。方法采用酶联免疫技术(ELISA法)检测88例进展期胃癌病人,根据术后病理证实伴淋巴结转移组50例,不伴淋巴结转移组38例,10例早期胃癌病人,10例胃溃疡病人术前血清VEGF-D的水平,并探讨其与进展期胃癌的侵袭与淋巴道转移的关系。结果进展期胃癌血清中VEGF-D的水平明显高于胃溃疡组(P<0.05),和早期胃癌组比较,差异无显著性(P>0.05)。在进展期胃癌病人中,随胃癌浸润深度的增加,浸润深度为T3-T4组明显高于T1-T2组(P<0.05)。低分化型癌的病人血清VEGF-C水平明显高于高、中分化型癌的病人(P<0.05)。而在性别、肿瘤位置、组织病理类型方面比较,差异无显著性(P>0.05)。伴淋巴结转移组血清VEGF-D的阳性率和不伴淋巴结转移组比较差异无显著性(P>0.05)。结论血清中高水平的VEGF-D与进展期胃癌的侵袭以及组织分化密切相关,与淋巴结转移无明显相关性。

Objective To determine serum level of vascular endothelial growth factor D and its relationship with invasion and lymph node metastasis in gastric carcinoma patients at advanced stage. Methods There 88 patients with gastric carcinoma were divided into positive lymph node metastasis group consited of 50 patients and negtive lymph node metastasis group consisted of 38 patients according to pathology after operation. The level of VEGF-D in 10 patients with early gastric carcinoma and 10 patients with gastric ulceration were assayed by using enzyme-labeled immunosorbent assay. Results The serum level of VEGF-D in AGC was higher obviously than gastric ulceration group （P〈0.05）but without significance in comarison with EGC（P〉0.05）. Soakage type in AGC was higher remarkably than ulceration type（P〈0. 05 ）, low tissue differentiation in AGC was much higher than that of high and middle tissue differentiation （P〈0.05）. But there were no statistical significance in gender,location of carcinoma, and pathology type （P〉0.05）. Compared with negtive group, positve rate of serum VEGF-D in positive lymph node group was no significance（P〉0.05）. Conclusion The high level of serum VEGF-Dis closely correlated with the invasion of AGC. But it is not associated with lymph node metastasis.