小儿感冒茶的薄层鉴别和定量测定研究

Thin layer chromatographic identification and quantitative determination of Xiao′er Ganmao Tea

目的为制定小儿感冒茶（山银花、板蓝根、重楼）的质量控制方法。方法用TCL鉴别山银花、板蓝根、重楼。用高效液相色谱法测定小儿感冒茶中绿原酸的含量。结果通过方法学考察，绿原酸检测浓度在0．244—1．952μg范围内与峰面积积分值呈良好的线性关系，加样回收率为99．22％，RSD％=0．92（n=2）。结论实验应用方法简便准确、重现性好、精密度高。所建质量标准可用于小儿感冒茶的质量控制。

Objective To establish a method for the quality control of Xiao＇er Ganmao Tea（herbal tea for pediatric cold with main ingredients including Flos Lontcerae Japonicae, Radix Isatidis and Rhizoma Paridis ）. Method The Flos Lontcerae Japonicae,Radix Isatidis and Rhizoma Paridis in the herbal tea were identifred by TLC and the content of Chloro- genic acid in the Tea was determined by HPLC. Result The linear range of chlorogenic acid was 0.244 - 1. 952 μg（r = 0. 9998）. The average recovery was 99.2% and the RSD was 0.92%. Conclusion The TLC method is simple and accurate with good reproducibility and high precision,which can be used for quality control of Xiao＇er Ganmao Tea.