针刀松解法对兔膝骨关节炎模型行为学和形态学的影响

Influences of acupotomy therapy on ethology and morphology of rabbit model of knee osteoarthritis

目的通过观察针刀松解法对兔膝骨关节炎模型行为学和形态学的影响,探讨骨性关节炎的发病机理及针刀松解法治疗的可行性。方法以膝关节伸直位固定法造成膝骨关节炎模型,用电针和针刀进行治疗,治疗3周后观察Lequesne MG的膝关节评估级别,取胫骨组织HE染色后进行光镜观察,切取股骨内髁软骨进行透射电镜观察。结果Lequesne MG评分显示在局部反应、步态反应和关节活动上,电针组和针刀组分别与正常组、模型组比有显著性差异(P<0.05),电针组和针刀组之间比较无显著性差异(P>0.05)。HE染色显示电针组和针刀组软骨组织损伤减轻,且有修复趋势;电镜结果显示针刀组兔膝关节软骨细胞破坏程度和胶原纤维紊乱程度明显减轻,与电针组相当。结论针刀松解法对膝骨关节炎有较好的治疗效果,从宏观到微观证实了针刀松解法的有效性。

Objective To investigate the pathogenesis of osteoarthritis and feasibility of acupotomy therapy through the observation on the influences of acupotomy therapy on ethology and morphology in rabbits with knee osteoarthritis. Methods The model of knee osteoarthritis was established by applying the fixed method of knee extension posture in rabbits. The therapy of electro-acupuncture and aeupotomy therapy were used to treat the rabbits. After 3 weeks the knee joint function was observed and reviewed based on Lequesne algofunctional index. The light microscope was used to observe tibia tissue after HE staining. The transmission electron microscope （TEM） was used to observe the internal femoral condyle cartilage. Results Lquesne algofunctional index showed that in local reaction, gait reaction and joint motion there was a significant different between the electro-acupuncture group or acupotomy group and the normal group or model group （P 〈 0.05 ）, respectively, while there was no different between the electro-acupuncture group and acupotomy group （P 〉 0.05 ）. HE staining showed that the cartilage injury was relieved and tended to recovery in the electro-acupuncture group and acupotomy group. The result of TEM showed that the lesion of cartilage cells and disorder of collagen fibrils were relieved in the aeupotomy group, which is similar to those in the electro-acupuncture group. Conclusion The acupotomy therapy has a good curative effect on knee osteoarthritis, which is proved at macroscopic and microscopic levels.