基因芯片荧光靶点阵列图像CCD扫描采集系统

Gene Chip Microarray Fluorescent Spots Images CCD Scanning and Acquiring System

为提高芯片图像扫描采集效率,研制了一种基因芯片荧光靶点阵列图像CCD扫描采集系统,通过将落射式荧光显微镜、紫外荧光激发光源、精密电控平移台和半导体制冷CCD相机等仪器进行有效集成构建成像扫描机构,以工业控制计算机(IPC)为测控核心,实现了显微镜自动调焦控制、荧光靶点图像自动扫描和采集、靶点阵列图像拼接等功能,提高了核酸扩增杂交反应基因芯片试验效率。

To promote the efficiency of microarray gene chip images scanning and acquiring, a microarray fluorescent spots images scanning system is developed, in which an epi-fluorescence microscope, a precision threedimensional （3D） translation motion platform, and a thermo electric cooling CCD camera are integrated effectively to construct an imaging and scanning mechanism. This system utilizes the industrial personal computer （IPC） as the core of measurement and control and realizes three specific functions, such as microscope auto focusing control, fluorescent spots images auto scanning and sampling and microarray spots images stitching. Many assays and experiments have been preformed to manifest that the system can effectively promote the efficiency of the microarray polymerase chain reaction （PCR） experiments and analysis.