菟丝子种子休眠原因及解除方法初探

Reason for dormancy of Cuscuta chinensis seed and solving method

目的:陈年菟丝子干种子常因进入次生休眠而影响发芽率,本实验探讨导致中药菟丝子休眠的主要原因,并寻求最佳处理方法。方法:单独处理与复配处理结合,运用正交设计,分析发芽实验结果。结果:陈年菟丝子干种子吸水能力良好;水浸液与甲醇提取物均有发芽抑制物,后者抑制作用较强;单独处理效果普遍不如复配处理。结论:种子抑制物的存在是种子休眠的主导因素,最佳的处理方法分别为:①浓硫酸处理2min后,用500mg·L-1的GA3溶液处理60min;②100mg.L-1的NaOH溶液处理20min后,用500mg·L-1的GA3溶液处理120min。与空白对照15.3%的发芽率相比,最高萌发率分别达到了43.3%,41.7%。

Objective ： To study the reason for the deep dormancy of the aged Cuscuta chinensis seed and find the solving method. Method： The separated and combined treatments were applied in the orthogonal designed experiments. Result： The aged seed had well water-absorbency ; the water and ethanol extracts of the seeds showed an inhibition effect on germination capacity of the seeds. Conclusion ： The main reason for the deep dormancy of aged C. chinensis seed is the inhibitors existed in seed. There arc two methods to solve the problem. The seeds is immersed in 98% of H2SO4 for 2 min followed by 500 mg ·L^-1 of GA3 treatment for 60 min, or in 100 mg ·L^-1 of NaOH for 20 min followed by 500 mg ·L^-1 of GA3 treatment for 120 min.