醒脑静中各味药物及全方对栀子提取物肠吸收的影响

Study of components in Xingnaojing affecting intestine absorption of gardenia extract

目的:考察醒脑静处方中艾片、麝香、郁金及合方后对栀子提取物肠吸收的影响。方法:采用大鼠在体肠吸收模型研究方中不同配伍条件下栀子提取物有效成分栀子苷的肠吸收情况。结果:栀子提取物质量浓度为0.8g·L-1(含栀子苷0.311g·L-1)时栀子苷吸收速率常数为(0.055±0.006)h-1(以栀子苷计);栀子提取物分别与艾片、郁金、麝香配伍时吸收速率常数分别为(0.072±0.010),(0.060±0.001),(0.066±0.008)h-1,合方后吸收速率常数为(0.076±0.011)h-1。经统计学分析,单味栀子组与配伍艾片组的吸收速率常数间有显著性差异(P=0.03),与配伍郁金和配伍麝香组的吸收速率常数间无显著性差异,与复方组的吸收速率常数间有显著性差异(P=0.02)。结论:配伍艾片及复方给药时可以显著促进栀子提取物中栀子苷的肠吸收。

Objective： To observe the influence of ingredients in Xingnaojing, such as moschus, borneol and radix curcumae on intestine absorption kinetics of gardenia extract. Method ： An in situ intestinal perfusion model of rats was employed to investigate the absorption of geniposide in gardenia extract. Result： While gardenia extract was administered solely, the absorptive rate constant （K） of geniposide was （0. 055 ±0. 006 ） h^-1 ; But while the extract was co-administered with radix curcumae, moschus and borneol, the Kwere （0.060±0.001）,（0.066 ±0.008）,（0.072±0.010） h^-1,respectively. The K was（0.076±0.011） h^-1 while the extract in total formulation for Xingnaojing. Conclusion： The K, while the extract is co-administered with borneol or total formulation is significantly higher than solely used. Borneol and complex prescription can significantly increase the intestinal absorption of geniposide in gardenia extract.