芦荟提取物有效成分对神经细胞和脑线粒体的保护作用

Protective effects of Aloe vera extract on mitochondria of neuronal cells and rat brain

目的:了解芦荟提取物有效成分(AV)对神经细胞和大鼠脑线粒体损伤的保护作用机制。方法:叠氮钠处理PC12细胞后,显微镜下观察细胞形态;MTT法检测细胞存活率;JC-1法检测PC12细胞线粒体膜电位变化。提取大鼠脑线粒体,刃天青法检测大鼠脑线粒体功能,TBA法检测大鼠脑线粒体MDA含量。结果:AV能显著改善叠氮钠引起的PC12细胞线粒体损伤,叠氮钠0.064mol·L-1作用4h,PC12细胞存活率降低47.8%,AV1,10mg·L-1均显著提高叠氮钠诱导的PC12细胞存活率降低,提高率分别为16.7%(P<0.05)和22.3%(P<0.01);AV1,10mg·L-1均可显著改善叠氮钠诱导的线粒体膜电位降低(P<0.05)。AV也可保护大鼠脑线粒体结构和功能的稳定,AV10mg·L-1显著改善叠氮钠诱导的线粒体的氧化还原功能损伤;AV1,10mg·L-1显著抑制Fe2+-cysteine诱导的大鼠脑线粒体中脂质过氧化物生成(P<0.05,P<0.01)。结论:芦荟提取物有效成分对神经细胞和大鼠脑线粒体损伤具有保护作用。

Objective： To investigate the effects of Aloe vera extract （AV） on mitochondria in rat pheochromocytoma （PCl2） cells and rat brain and to study the mechanism of its neuroprotection. Method： After treatment, the morphology of PCl2 cells was observed under microscope, the activity of mitochondria in PCl2 cells was measured by MIT method, and the mitochondrial membrane potential （MMP） in PCl2 cells was detected by JC-1 method. The mitochondrial function in rat brain was detected by resazurin method. The production of malondialdehyd （MDA） in rat brain mitochondria was tested by thiobarbaturic acid （TBA） assay. Result： AV could improve mitochondrial damage induced by azide sodium （ NaN3 ） in PC12 cells. The viabihty of PC12 cells treated with NAN364 mmol·L^-1 for 4 h decreased by 47.8% , and AV at 1 and 10 mg·L^-1 could respectively increase the viability of NaN3-treated cells by 16. 7 % （ P 〈 0. 05 ） and 22.3 % （ P 〈 0. 01 ）. MMP in PC 12 cells in AV 1 and 10 mg·L^-1 group was significantly higher than that of NaN3-treated group （P 〈0. 05）. AV also protected the structure and function of mitochondria in rat brain. AV at 10 mg·L^-1 had protective effect on mitochondria function impair induced by NaN3 （ P 〈 0. 01 ）. AV 1 and 10 mg·L^-1 markedly inhibited the lipid peroxidation of brain mitochondria induced by Fe^2＋ -cysteine （ P 〈 0. 05, P 〈 0. 01 ）. Conclusion ： AV has protective effects on mitochondria of neuronal cells and rat brain.