红花桑寄生多糖抑制小鼠S180肉瘤生长的研究

Polysaccharides from Scurrula parasitica L. inhibit sarcoma S180 growth in mice

目的:研究红花桑寄生多糖的体内外抑瘤作用。方法:采用水提醇沉法提取分离红花桑寄生多糖(Scurrula para-sitica polysaccharides,SP),MTT法检测了SP对S180,K562和HL-60细胞增殖的抑制作用,腹腔给药检测SP对S180的体内抑瘤效果,并采用免疫组化方法分析了SP对瘤组织中Ki-67,Cyclin D1,Bax和Bcl-2等细胞增殖与凋亡抗原表达的影响。结果:SP体外无抑瘤作用,体内抑制S180实体瘤生长的最适剂量为100mg·kg-1.d-1,抑瘤率为54%,SP对小鼠体重增长无影响。SP可下调瘤组织中Ki-67,Cyclin D1和Bcl-2抗原的表达,上调Bax的表达。结论:SP可通过体内途径抑制瘤细胞增殖和促进瘤细胞凋亡而起到抗肿瘤作用。

To study the anti-tumor activity of Scurrula parasitica polysaccharides （SP）. Water extraction and ethanol precipitation were used to isolate SP from S. parasitica leaf. S180, K562 and HL-60 cell lines proliferation inhibition by SP were detected by MTT assay. The expressions of Ki-67, Cychn D1, Bax and Bcl-2 protein in the sarcoma S180 tissues were detected by immunohistochemistry technique to approach the anti-tumor mechanism of SP ＋ SP could not inhibit cancer cell proliferation. SP ip could inhibit the growth of sarcoma S180 in mice, 100 mg·kg^-1·d^-1. SP ip was the optimal dose on inhibiting S180 growth, with the tumor inhibition rate of 54%. The expression of Ki-67, Cyclin D1, Bax and Bcl-2 protein in the sarcoma S180 tissues were detected by immunohistochemistry technique to approach the anti-tumor mechanism of SP. The result showed that SP could down-regulate the expression of Ki- 67, CyclinD1 and Bcl-2 protein, and up-regulate the expression of Bax protein. It indicted that inhibiting cancer cell proliferation and promoting cancer cell apoptosis in vivo maybe one of the anti-cancer mechanisms of SP.