藕节炭的TLC鉴别和浸出物含量测定

Identification of Charred Nelumbo nucifera by TLC and Content Determination of Extract

目的:对藕节炭药材的鉴别方法及浸出物进行研究。方法:以3-表白桦脂酸、β-谷甾醇为对照,采用薄层色谱(TLC)法对藕节炭药材进行定性鉴别;采用65%乙醇为溶剂测其浸出物的含量。结果:TLC斑点明显;浸出物测定方法可行。结论:TLC法可以鉴别藕节炭药材;不同地区市售藕节炭65%乙醇浸出物含量差异较大。

OBJECTIVE： To study the identification method of charred Nelumbo nucifera and its extract. METHODS： The identification of charred Ni nucifera was carried out by TLC with 3-epibetulinic acid and β-sitosterol as reference substances. The extract was determined with 65% alcohol as solvent. RESULTS： The TLC spots were clear and determination method was feasible. CONCLUSION： TLC can be applied to determine charred N. nucifera and the contents of 65% alcoholic extracted from charred N.nucifera were different place by place.