RP-HPLC法测定小儿咳喘口服液中氨茶碱、麻黄碱和愈创木酚甘油醚的含量

Determination of Aminophylline,Eyhedrine and Guaifenesin in Xiaoer Kechuan Oral Solution by RP-HPLC

目的:建立以反相高效液相色谱法测定小儿咳喘口服液中氨茶碱、麻黄碱和愈创木酚甘油醚含量的方法。方法:色谱柱为Hypersil ODS(150mm×4.6mm,5μm),流动相为乙腈∶0.015mo·lL-1磷酸二氢钾溶液(含0.1%三乙胺,磷酸调pH2.7)=10∶90,检测波长为254、209nm,流速为1.6mL·min-1。结果:氨茶碱、麻黄碱、愈创木酚甘油醚的检测浓度分别在356.4～712.8、88～176、106～212μg·mL-1范围内与各自峰面积积分值呈良好的线性关系(r均为0.9999);平均回收率分别为101.0%、99.7%、98.3%,RSD分别为0.73%、1.00%、0.44%(n均为9)。结论:本法简便、准确,可用于小儿咳喘口服液的质量控制。

OBJECTIVE ： To establish an RP-HPLC method for the determination of aminophylline, eyhedrine and guaifenesin in Xiaoer kechuan oral solution. METHODS： The determination was performed on Hypersil ODS coltmm（150 mm×4.6mm, 5 pm）. The mobile phase consisted of acetonitrile-0.015 mol. L-1 KH2PO4（0.1% triethylamine and pH value of phosphoric acid adjusted to 2.7） （10：90） with a flow rate of 1.6 mL.min-1 and detection wavelength of 254 nm and 209 nm. RESULTS： The linear ranges were 356.4-712.8μg.mL-1 for aminophyUine, 88-176 μg.mL-1 for eyhedrine and 106-12 μg.mL-1for guaifenesin（r=0.999 9）. The average recovery rates were 101.0% （RSD=0.73% , n=9） , 99.7% （RSD=1.00% , n=9） , 98.3% （RSD=0.44% , n=9） , respectively.CONCLUSIONS：The method is simple and accurate for quality control of Xiaoer kechuan oral solution. KEY WORDS RP-HPLC; Xiaoer kechuan oral solution; Aminophylline; Eyhedrine; Guaifenesin; Content determination