RP-HPLC法测定维感泡腾片中腺苷的含量

Determination of Adenosine in Weigan Effervescent Tablets by RP-HPLC

目的:采用RP-HPLC法建立维感泡腾片中腺苷的含量测定方法。方法:以甲醇-水(8∶92)为流动相,ShimadzuC18(250 mm×4.6 mm,S-5μm)色谱柱,检测波长为260 nm,柱温为35℃,流速为1.0 mL.min-1。结果:腺苷在4.3~33.8μg范围内呈良好的线性关系(r=0.999 7),腺苷的平均回收率为101.0%(n=9,RSD=1.4%)。结论:该方法快速简便易行,重复性良好,结果准确,可作为维感泡腾片中腺苷质量评价的方法。

Objective：To develop a RP-HPLC method for the quantification of adenosine in Weigan Effervescent Tablets.Methods： The analysis was carried out on a Shimadzu C_（18） column（250 mm×4.6 mm,S-5 μm）and the mobile phase was a mitture of methanol-water（8∶92）with a flow rate of 1.0 mL· min^（-1）.The detection wavelength was 260 nm,and the column temperature was 35 ℃.Results： The linear response ranges of adenosine was 4.3～33.8 μg（r=（0.999 7））,and the average recovery was 101.0%（n=9,RSD=1.4%）.Conclusion： The method is quick,simple and reproducible for the determination of adenosine in Weigan Effervescent Tablets.