黄连吴茱萸药对水提物对乙醇致大鼠胃损伤的保护作用

Protective Effects of Water Extract of Rhizoma Coptidis and Fructus Evodiae on Ethanol-induced Rat Gastric Lesions

目的:研究不同配比黄连吴茱萸药对水提物对乙醇致大鼠急性胃损伤的保护作用及其可能的机制。方法:大鼠经腹腔注射6∶1,6∶6,1∶6 3种不同配比的黄连吴茱萸药对水提物后,通过形态测量法测定胃损伤程度;用ex-vivo chamber法测定50%乙醇造成胃损伤前后的胃电压(PD)变化及灌流液pH值和H+含量。结果:与对照组比较,黄连吴茱萸1∶6药对水提物显著降低胃损伤指数,加快PD的恢复,降低胃灌流液pH值和增加H+含量;黄连吴茱萸6∶6降低胃损伤指数。结论:黄连吴茱萸1∶6水提物对50%乙醇造成的大鼠急性胃损伤具有较好的保护作用,显著优于黄连吴茱萸6∶1和6∶6;其机制可能与抑制酸反渗,增强胃黏膜屏障功能有关,提示吴茱萸在介导胃黏膜保护作用中担当着重要的作用。

Objective：To investigate the protective effects of water extract of Coptis chinensis and Evodia rutaecarpa on ethanol-induced acute gastric lesions in rats.Methods：In this study,animals were intraperitoneally pretreated with vehicle（normal saline） or Coptis chinensis and Evodia rutaecarpa water extract at 1050（6∶1）,1800（6∶6）,1050（1∶6） mg·kg^（-1）.Three hours later,gastric lesions were induced by topical application of 50% ethanol for 10 min.The length index of gastric lesions were determined by morphological analysis in a gastric intubatton model.The rat gastric transmucosal potential difference（PD） was recorded continuously by ex-vivo chamber technique.The pH value and H~＋ content of gastric perfusate were mesured by pH meter and pH-stat technique,respectively.Results：Compared with control,Coptis chinensis and Evodia rutaecarpa water extract（1∶6） accelerated PD recovery and reduced gastric morphologic lesions.Coptis chinensis and Evodia rutaecarpa water extract（1∶6） elevated H~＋ content and reduced pH value.Coptis chinensis and Evodia rutaecarpa water extract（6∶6） reduced gastric morphologic lesions.Conclusions：Coptis chinensis and E.rutaecarpa water extract（1∶6） showed effectively protective actions on ethanol-induced acute gastric lesions in rats,and the gastroprotective mechanisms maybe due to the strengthening action on gastric mucosal lining and the inhibition of acid backdiffusion.This investigation also indicated that E.rutaecarpa maybe have a critical role in gastroprotective effect.