摘要
以含有鸡传染性支气管炎病毒Ｈｏｌｔｅ株Ｓ１基因的重组质粒ｐＵＣ１８Ｓ１．Ｈｏｌｔｅ和ｐＵＣ１８Ｓ′１．Ｈｏｌｔｅ为材料，分别构建了可表达ＩＢＶＳ１基因的昆虫杆状病毒转移载体质粒ｐＳＸＩＶＶＩ＋Ｘ３Ｓ１．Ｈｏｌｔｅ（含ＩＢＶ先导序列）、ｐＳＸＩＶＶＩ＋Ｘ３／４Ｓ１．Ｈｏｌｔｅ（ＩＢＶ先导序列作为融合短肽）、ｐＡｃＧＨＬＣＳ１．
The recombinant transfer plasmids pSXIVVI +X3 S1.Holte and pSXIVVI +X3/4 S1.Holte and pAcGHLT C S1.Holte were constructed by insertion of the cDNA of the S1 gene of avian infectious bronchitis virus (IBV) into transfer vector plasmids pSXIVVI +X3 and pSXIVVI +X3/4 and pAcGHLT C.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1998年第4期1-4,共4页
Journal of South China Agricultural University
基金
国家教委高校博士点基金
关键词
S1基因
杆状病毒
表达载体
构建
IBV
鸡
avian infectious bronchitis virus(IBV)
S1 gene
baculovirus expression vector
construction
