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Identification of superior clones by RAPD technology in Xanthoceras sorbifolia Bge. 被引量:4

Identification of superior clones by RAPD technology in Xanthoceras sorbifolia Bge.
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摘要 It is very important to select and identify superior Xanthoceras sorbifolia Bge. trees for purposes of breeding and exploration. For our study, we selected superior X. sorbifolia trees using RAPD (random amplification of polymorphic DNA) technology. The results show that certain specific amplification bands emerged with significantly different frequencies between superior and non-superior trees. The 1400-bp band of No. 2121 primer emerged in the superior trees at a frequency 2.4 times that of the non-superior trees and the 600-bp band of No. 2153 primer in the non-superior trees at a frequency 2.5 times that of the superior trees There were very significant differences between the superior and non-superior trees. The frequency of the 1600-bp band of No. 1161 primer was the same in both kinds of trees, but significant differences were observed in point frequency (the frequency of the superior trees is 1,4 times that of the non-superior trees). These bands can be used as important targets to distinguish between superior and non-superior trees. The use of a combination of amplified bands simultaneously can play a guiding role in the selection of superior X. sorbifolia trees. It is very important to select and identify superior Xanthoceras sorbifolia Bge. trees for purposes of breeding and exploration. For our study, we selected superior X. sorbifolia trees using RAPD (random amplification of polymorphic DNA) technology. The results show that certain specific amplification bands emerged with significantly different frequencies between superior and non-superior trees. The 1400-bp band of No. 2121 primer emerged in the superior trees at a frequency 2.4 times that of the non-superior trees and the 600-bp band of No. 2153 primer in the non-superior trees at a frequency 2.5 times that of the superior trees There were very significant differences between the superior and non-superior trees. The frequency of the 1600-bp band of No. 1161 primer was the same in both kinds of trees, but significant differences were observed in point frequency (the frequency of the superior trees is 1,4 times that of the non-superior trees). These bands can be used as important targets to distinguish between superior and non-superior trees. The use of a combination of amplified bands simultaneously can play a guiding role in the selection of superior X. sorbifolia trees.
出处 《Forestry Studies in China》 CAS 2010年第1期37-40,共4页 中国林学(英文版)
基金 supported by a Project of the State Forestry Administration and the PetroChina Company Limited Lin-you Integrated Bio-diesel Raw Forest Base
关键词 Xanthoceras sorbifolia Bge. superior clones RAPD technology Xanthoceras sorbifolia Bge., superior clones, RAPD technology
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