摘要
目的探讨补肾壮骨方药对骨癌疼痛模型痛骨质破坏的治疗作用。方法SD大鼠左后肢胫骨内接种MRMT-1细胞制成骨转移癌疼痛模型，给予补肾壮骨方药治疗。观察大鼠痛行为学改变，包括von Frey纤维刺激的50％缩足阈（50％PWT）和热刺激缩足潜伏期（TWL）。对胫骨标本用HE染色观察病理形态学改变。结果本研究采用骨癌疼痛模型出现肿瘤生长、骨质破坏和癌性骨痛等表现。相对于模型组，各中药治疗组50％PWT明显延长、TWL延长。病理形态学显示骨质破坏改善，肿瘤增殖减少。结论补肾壮骨方药可有效治疗骨转移癌疼痛，抑制骨转移肿瘤灶，减轻骨转移所致的溶骨性骨质破坏。
Objective To evaluate therapeutic effects of Bushen-Zhuanggu medicine extraction on bone destruction in rats bone cancer pain. Methods Rat models of cancer-induced bone pain were established by inoculating MRMT-1 cells into tibia of rats. Behavioral signs indicative of pain including 50% paw withdrawal threshold （yon Frey tactile sensitivity test） and thermal withdrawal latency were observed. Pathomorphological changes of tibia were monitored with HE staining. Results In the behavioral tests, herbal medicine treatment attenuated mechanical allodynia and thermal hyperalgesia. Histological examination showed that this treatment inhibited tumor proliferation and preserved the cortical and trabecular bone structure. Conclusion Bushen-Zhuanggu medicine extraction is an anti-nociceptive and bone-preserving agent in rats of bone cancer pain.
出处
《国际中医中药杂志》
2010年第1期7-8,共2页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目（项目编号：30772858）
关键词
骨转移癌疼痛
补肾壮骨方
骨质破坏
Bone cancer pain
Bushen-Zhuanggu medicine extraction
Bone destruction
