马缨杜鹃下胚轴组织培养及全息现象的研究

Research on the Tissue Culture and the Holographic Phenomenon of Rhododendron delavayi Franch.Hypocotyl

以马缨杜鹃下胚轴为外植体,用L9(34)正交实验法研究基本培养基、NAA、2-ip对芽器官诱导的影响。以马缨杜鹃下胚轴切断为材料,研究全息现象。结果表明马缨杜鹃下胚轴诱导芽的最佳培养配方为1/4 MS+NAA0.75 mg/L+2-ip2.0 mg/L。马缨杜鹃切段在离体培养时存在全息现象,其不定芽发生的部位及数量、频率的梯度变化都遵循生物全息律,全息律的呈现在一定程度上受外源激素和切断位置的影响。

Using Rhododendron delavayi Franch hypocotyl as explant, through the L9 （ 3^4 ） orthogonal experi- ment, a study was conducted to study the effect of tissue culture on organs sprouting by basic culture medium, NAA,2-ip. Moreover, researched bioholographic phenomenon by hypocotyl cutting tissue culture. Results showed that the optimal combination of bud differentiation was 1/4 MS ＋0.75 mg/L NAA ＋2-ip 2.0 mg/L. It was found that in vitro culture of hypocotyl segments showed holographic phenomenon; the position and the quantity ,the frequency gradient of sprouting inducing all conformed to the bioholographic law ,the hormone and the position of hypocotyl could effect the bioholographic law to a certain extent.