刺五加种子休眠解除过程中过氧化物酶和酯酶同工酶的变化

Changement of Peroxidase and Esterase Isozymes During Seed Dormancy-breaking Period of Acanthopanax senticosus Harms

剌五加种子用100 mg/L赤霉素(gibberellin,GA)浸泡后进行变温层积(15～18℃14周,2～6℃14周)处理。层积16周后,种子开始萌发,GA处理种子的萌发率明显高于对照,在28周时,GA处理的萌发率达到69.33%,而对照只有50%。在实验期间,聚丙烯凝胶电泳始终能够检测到1条过氧化物酶(peroxidase,POD)和1条酯酶(esterase,EST)同工酶带,GA处理促进这两条同工酶带的表达。与对照相比,GA处理使层积16周出现的POD新条带更强,使EST新条带提前(第12周)出现(对照在第16周)。结果表明,外源GA处理促进层积打破刺五加种子休眠的效应,GA的这种效应与促进POD和EST同工酶的基因表达有关。

The seeds of Acanthopanax senticosus Harms were soaked in 100 mg/L gibberellin （GA） solution and then treated with fluctuating stratification. The seeds began to germinate after stratifying 16 weeks,germi- nation percentage of the GA-treated seeds was significantly higher than the control. After 28 weeks, germination rate of the GA-treated seeds reached to 69.33%, in contrast, the control only 50%. During the experiment, polyacrylamide gel electrophoresis （PAGE） detected a band of peroxidase （POD） isozyme and a band of es- terase （EST） isozyme at all stages ,and GA could promote the two kinds of isozyme to express. Compared with the control,GA could make the new band of POD become stronger after stratificating 16 weeks and the new band of EST appeared first. The results showed that exogenous GA treatment enhanced the dormancy-breaking effect on stratification of Acanthopanax senticosus Harms seeds, and the effect of GA was related to the gene expression of POD and EST.