神经生长因子对瘢痕成纤维细胞影响的实验研究

The experiment study of NGF on scars fibroblasts

目的:探讨神经生长因子(NGF)对瘢痕成纤维细胞生物学行为的影响、病理改变及其对瘢痕的作用机制,为临床预防和治疗瘢痕提供分子及病理学水平的理论依据。方法:对大鼠动物创伤模型进行体内实验,采用倒置显微镜、HE染色、Cason氏改良三色染色、Hamason-Lush-bangh氏染色法、透射电镜等方法进行观察。结果:大鼠动物创伤模型病理染色与透射电镜观察显示,与对照组相比,实验组局部注射细胞因子NGF后,促进了成纤维细胞的生长增殖,刺激了胶原纤维的合成、增生和沉积。结论:NGF对成纤维细胞的生长趋势与增殖活性有明显的促进作用,刺激了胶原纤维的合成、增生和沉积。

Objective：To explore the effects of NGF on the fibroblasts of scars in biological behavior and pathological change. It can provide the basic of theory in molecular biology and pathobiology. Methods： Wounds were created on a Sprague-Dawley （SD） rat model. The results were observed and analysed by light inverted microscope（LM）, transmission elextron microscope （TEM）, HE staining, Cason, s staining and flow cytometry（FCM）. It was determined that effects of NGF on scars in biological behavior and pathological change. Results： In vivo（rat model） ：using pathological staining and TEM, eompared to the controls, it could be obtained that the fibroblast proliferation was enhanced and collagen synthesis was increased by topically applying NGF. Conelusions：NGF can inerease fibroblasts proliferation and collagen synthesis, seeretion and accumulation.