罗格列酮和双酚A型环氧树脂对脂多糖应激断奶仔猪白细胞分类计数和血液生化指标的影响

Effects of Rosiglitazone and Bisphenol A Diglycidyl Ether on White Blood Cell Differential Count and Blood Biochemical Indexes of Weaned Piglets with Lipopolysaccharide Challenge

试验研究了过氧化物酶体增殖物激活受体γ(PPARγ)的激动剂和抑制剂对脂多糖(LPS)应激断奶仔猪白细胞分类计数和血液生化指标的影响。选取24头健康断奶仔猪,分为4组,每组6个重复,每个重复1头猪。4个组分别为:对照组;LPS组;罗格列酮(ROS,PPARγ激动剂)+LPS组;双酚A型环氧树脂(BADGE,PPARγ抑制剂)+ROS+LPS组。注射LPS或生理盐水3 h后,前腔静脉采血待测。结果显示:1)ROS缓解了LPS导致的断奶仔猪血液白细胞总数和淋巴细胞数量的降低(P<0.05),BADGE阻断了ROS的缓解作用(P<0.05);2)ROS缓解了LPS导致的断奶仔猪血浆碱性磷酸酶活性的升高(P<0.05)及总蛋白、白蛋白和球蛋白含量的降低(P<0.10),BADGE阻断了ROS的缓解作用(P<0.10)。结果表明,ROS在一定程度上缓解LPS应激导致的断奶仔猪白细胞总数和淋巴细胞数量的下降及部分血液生化指标的改变,而BADGE阻断了ROS的缓解作用。

The experiment was conducted to investigate the effects of PPARγ, agonist or antagonist on white blood cell differential count and blood biochemical indexes of weaned piglets with lipopolysaccharide （LPS） challenge. Twenty-four weaned piglets were allotted to 4 groups （6 replicates per group and 1 piglet each replicate）, including control group, LPS group, rosiglitazone （ROS, PPARγ, agonist） ＋ LPS group and bisphenol A diglycidyl ether （BADGE, PPAR γ antagonist） ＋ ROS ＋ LPS group. Blood samples were collected at 3 h post-challenge of LPS. The results showed as follows： 1） ROS alleviated the decrease of white blood cells and lymphocytes counts of weaned piglets induced by LPS （P〈0.05）. BADGE inhibited the effects of ROS on white blood cells （P〈0.05） ; 2） ROS alleviated the increase of alkaline phosphatase activity （P〈0.05） and the decrease of total protein, albumin and globulin content （P〈0.10） of weaned piglets induced by LPS. And BADGE inhibited the effects of ROS （P〈0.10）. These results indicated that ROS alleviated the decrease of white blood cells and lymphocytes counts and change of some blood biochemical indexes of weaned piglets induced by LPS to some degree, and BADGE blocked the effects of ROS.