乙型肝炎肝组织中Fas抗原表达的研究

Study on expression of Fas in liver tissue of patients with hepatitis B

目的:探讨乙型肝炎肝组织Fas抗原表达与细胞凋亡和肝细胞损伤关系.方法:用免疫组织化学技术,对109例乙型肝炎肝组织Fas抗原进行了检测.结果:Fas抗原总检出率76.16%,急性重症肝炎(AFH),慢性迁延型肝炎(CPH),慢性活动型肝炎(CAH),活动性肝硬化(AC)Fas抗原检出率分别为90.9%,43.48%,78.12%,85.71%.急性重症肝炎Fas抗原阳性细胞分布于大片坏死区域中残留肝细胞内,慢性肝炎和活动性肝硬化Fas抗原阳性细胞分布在碎屑状坏死灶周围或假小叶周边肝细胞.Fas抗原阳性率经统计学处理,CPH组与AFH,CAH及AC组有显著性差异.结论:Fas抗原在病毒性肝炎时有介导细胞凋亡的作用,其表达与肝细胞损伤程度密切相关.

Aims: To explore the relationship between the expression of Fas antigen and apoptosis and liver cell injury in liver tissue of patient with hepatitis B. Methods: Immunohistochemistry was used to i-dentify the expression of Fas antigens in the liver tissue specimens from 109 patients with hepatitis B. Result:The total rate of Fas positivity was 76.16%, with the positive rates of 90.9%, 43.48% , 78.12%and 85.71% for acute fulminant hepatitis(AFH). chronic persistent hepatitis (CPH), chronic active hepatitis (CAH) and active cirrhosis (AC) respectively. In AFH, the Fas antigen was found in the residual cells in the extensive necrotic regions. In CH and AC, Fas antigens were expressed in the cytoplasm of liver cells. Fas positive liver cells were distributed on the edges of piecemeal necrotic foci and pseudolobules. Statistics showed the Fas positive rate of CPH was significantly different from those of AFH, CAH and AC (x2=8.54,6.94, and 5.21). Conclusions; Fas antigen mediates apoptosis of liver cells and Fas antigen expression is closely related to liver cell injury due to hepatitis B.