痰热清注射液对脓毒症大鼠免疫调节及生存率的影响

Effect of Tanreqing Injection on Immunoregulation and Survival Rate of Rats with Sepsis

目的研究痰热清注射液对脓毒症大鼠免疫调节及生存率的影响。方法采用盲肠结扎穿孔术(CLP)制备脓毒症模型。112只Wistar大鼠中16只随机分为模型组及痰热清治疗组观察大鼠术后7d生存率;另96只大鼠按随机数字表法分为正常组、假手术组、模型组及痰热清治疗组。用ELISA法检测各组血浆降钙素原(PCT)和肿瘤坏死因子-α(TNF-α)水平。结果痰热清治疗组7d生存率明显高于模型组。与假手术组比较,模型组大鼠血浆PCT及TNF-α水平在CLP后6h明显升高,12h达到高峰,除2h时点外,各时点与假手术组比较差异有统计学意义;与模型组比较,除2h时点外,痰热清治疗组PCT及TNF-α水平明显降低。结论痰热清注射液可明显抑制脓毒症大鼠血浆PCT及TNF-α水平,提高脓毒症大鼠的生存率。

Objective： To explore the effect of Tanreqing Injection on immunoregulation and survival rate of rats with sepsis. Methods： According to CLP method, 112 Wistar rats were selected to be the sepsis models. 16 rats among them were randomly divided into the model group and the Tanreqing Injection group to observe the survival rates at postoperative 7 days. The other 96 rats were randomly divided into 4 groups as follows： the sham operation group, the normal control group, the model group and the Tanreqing Injection group. The levels of PCT and TNF -α in every group were examined by ELISA method. Results： Compared with the model group, the survival rates of rats in Tanreqing Injection group at 7 days were notably raised. Compared with the sham operation group, The levels of PCT and TNF-α in the model group were increased significantly at 6h after CLP, and then peaked at 12h after CLP. Except 2h, there were statistic differences between the Tanreqing Injection group and the sham operation group in other time points, every time points. Meanwhile, levels the of PCT and the TNF -α in the Tanreqing Injection group were induced significantly in these time points. Conclusion： Tanreqing Injection could not only obviously decrease the PCT and the TNF -α of rats with sepsis, but also the survival rate of these rats.