某自来水厂水源水有机提取物的雌激素样活性研究

Study on the Estrogenic Effects of Organic Extracts from Raw Water of a Waterworks

[目的]了解某自来水厂水源水有机提取物的雌激素样活性。[方法]用固相萃取法富集水样,分低、中、高剂量染毒组(体外试验为每毫升染毒量分别相当于10、100、1000mL水源水中有机提取物的剂量;体内试验为每千克体重灌胃量相当于2、5、10、25L水源水中有机提取物的剂量)、阴性对照组和雌二醇阳性对照组,通过体外人乳腺癌(MCF-7)细胞增殖试验、报告基因试验和初断乳大鼠子宫增重试验对水源水有机提取物的雌激素样活性进行研究。[结果]体外细胞增殖试验中,高剂量组较阴性对照组出现细胞增殖效应(P<0.05),细胞增殖率为(130.85±13.68)%;而报告基因试验中,中剂量组检测到荧光强度增加(P<0.05),相对荧光强度为(207.67±12.40)%。子宫增重试验中,相当于低、中、高剂量染毒组大鼠子宫湿重和黏液重脏器系数与阴性对照组相比差异无统计学意义。[结论]体外试验表明水源水中含有雌激素样活性物质,但体内试验尚不能确定该水源水具有明确的雌激素样活性。

[ Objective ] To study the estrogenic effects of organic extracts from raw water of a waterworks. [ Methods ] The estrogenic effects of organic compounds which were extracted from raw water by solid phase extraction method were detected by MCF-7 cell proliferation assay, luciferase reporter gene assay and rodent uterotrophic bioassay. The exposure doses were equivalent to making organic extractions from 10, 100, 1000mL raw water condensed into 1 mL ethanol in vivo, and from 2, 5, 10, 25 mL cindensed into 1 mL ethanol in vivo. [ Results ] Compared with the negative control group, the significant cell proliferation effect was detected in the group of 1000mL water sample, corresponding proliferation rate was（ 130.85 ± 13.68 ）%. The luciferase activity in 100 mL water sample group increased significantly, relative fluorescence intensity was （ 207.67 ± 12.40 ）%. Uterotrophic bioassay showed the relative weight of uterus and luminal contents of the organic extracts treated groups slightly increased compared with that of the vehicle control group, but the differences were not so significant. [ Conclusion ] In vitro assays showed that organic extracts from this raw water contained estrogen-like mixture, while in vivo assay didn＇t show definite estrogenic effects.