氯化铝致原代培养小鼠神经细胞毒性作用及对mGluR3表达的影响

The Toxic Effect of Aluminum Chloride on Primarily Cultured Mice Neurocytes and Gene Expression of mGluR3

[目的]探讨铝对原代培养神经细胞的毒性作用及对代谢型谷氨酸受体3(metabotropic glutamate receptors,mGluR3)基因表达的影响。[方法]采用结晶氯化铝(AlCl3·6H2O)分别对原代培养昆明小鼠的神经细胞染毒,使其终浓度分别为0mmol/L(对照组)、0.5mmol/L(低剂量组)、1.0mmol/L(中剂量组)和2.0mmol/L(高剂量组),染毒48h后,采用CCK-8试剂盒,使用酶联免疫仪检测细胞活力,采用逆转录-聚合酶链反应(RT-PCR)法测定mGluR3基因的表达。[结果]与对照组比较,高剂量组细胞活力明显下降(P<0.05);RT-PCR测定结果显示高剂量组相对于对照组mGluR3表达量明显降低(P<0.05)。[结论]铝对原代培养的神经细胞产生细胞毒性可能与mGluR3基因表达降低有关。

[ Objective ] To investigate the toxic effects of aluminum on primary cultured neurocytes in mice and the metabotropic glutamate receptor 3 （ mGluR3 ） expression. [ Methods ] The neurocytes were treated with aluminum chloride （ AlCl3 · 6H2O ）at final concentrations of 0 mmol/L（ control ）, 0.5 mmol/L（ low-dose ）, 1.0 mmol/L（ medium-dose ）, and 2.0 mmol/L （ high-dose ） separately; then incubated for 48 hour. The CCK-8 enzyme-linked immunosorbent assay kit was used to detect celt viability, and the expression ofmGluR3 were detected by RT-PCR. [ Results ] Compared with control group, AlCl3 decreased the viability of cultured neurocytes in high dose group significantly（ P 〈 0.05 ）. The RT-PCR demonstrated that mGluR3 gene expression in high dose group decreased significantly compared with control group after aluminum exposure（ P 〈 0.05 ）. [ Conclusion ] The cytotoxic effect of aluminum on primary cultured neurocytes in mice may be associated with the decrease of mGluR3 gene expression.