小麦-簇毛麦易位系6VS/6AL的RGAP分析

RGAP Analysis of Triticum aestivum-Haynaldia villosa 6VS/6AL Translocation Lines

根据抗病基因保守序列合成7对RGAP引物扩增携带Pm 21基因的小麦-簇毛麦易位系6VS/6AL和感病品种Chancellor,结果只有R11R/R11F引物对在6VS/6AL中扩增出1 317 bp的多态性片段。在其他抗白粉病基因载体品系中检测不到该片段。回收、克隆、测序结果的Blast分析表明,该片段第1-199核苷酸、第520-792核苷酸2个区域与GenBank中的已知序列有较高的类似性,这些序列中均含有抗性蛋白的保守区域。与小麦抗叶锈病基因Lr10的序列(AY270159.1)比较结果显示,两区域的类似性达89%和86%。发现由该片段推定的氨基酸序列包含抗病基因的类似序列,有1个不完整的核苷酸结合位点(NB-ARC)。

Based on the conserved sequence of resistance gene,seven pairs of RGAP primers were designed for RGAP analysis. The primer pair R11R/R11F amplified a special fragment from the 6VS/6AL line, but did not from other lines tested. Then the fragment was cloned, sequenced and analyzed by blast. The result showed that 1-199 and 520-- 792 nucleotide domains of the fragment had higher homology with known sequences in GenBank, which all included the conserved domains of resistance protein. The two domains had 89% and 86%, respectively, with the rele- vant sequence of wheat leaf rust resistance gene LrlO （AY270159.1）. The-deduced amino-acid se- quence contained the analog sequence of resistance gene, an incomplete NB- ARC domain.