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尿巨细胞病毒定量检测在婴儿巨细胞病毒感染诊治中的意义 被引量:5

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摘要 目的探讨荧光定量聚合酶链反应(FQ-PCR)法检测尿巨细胞病毒(CMV)在婴儿CMV感染的诊断与治疗动态监测中的应用价值。方法采用FQ-PCR法检测怀疑CMV感染婴儿血、尿CMV-DNA,分析阳性检出率、病毒含量及治疗后的病毒含量变化。同时与ELISA法检测CMV-IgM结果进行比较。结果396例婴儿中,FQ-PCR法血、尿阳性检出率分别为10.61%和61.87%,尿阳性检出率远高于血(P<0.01);ELISA法阳性率为31.06%,尿阳性检出率明显高于血。治疗前后尿CMV拷贝数均高于血,治疗后血、尿CMV拷贝数较治疗前明显下降。结论FQ-PCR法检测尿CMV在婴儿CMV感染的诊断与治疗动态监测中有良好的应用价值。
作者 唐娟 李东明
出处 《现代中西医结合杂志》 CAS 2010年第7期869-870,共2页 Modern Journal of Integrated Traditional Chinese and Western Medicine
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