壳聚糖金属离子配合物蛋白质印迹聚合物的制备与表征

The Preparation and Characterization of Protein Imprinting Polymer of Chitosan and Metal Ion Coordination Compound

利用壳聚糖与金属离子形成的配合物为功能单体,以环氧氯丙烷为交联剂,在模板分子牛血清白蛋白的存在下,采用简单方便的滴加成球法制备出牛血清白蛋白印迹聚合物。通过环境扫描电镜对其形貌结构进行了表征,并用蛋白质吸附实验对其印迹效果进行了评价。测试结果表明,用该方法制备的印迹聚合物对牛血清白蛋白具有较好的选择性,对模板蛋白质的吸附量是非印迹聚合物的21.9倍,显示出较明显的选择性吸附分离效果。

A molecular imprinting polymer was formed using bovine serum albumin as ＂print＂ molecule, epoxy chloropropane as crosslinker, and the coordination compound of chitosan and metal ion as functional matrix. The morphologies of the molecular imprinting polymer were characterized by environmental scanning electron microscope, and the selective adsorption and separation effect was evaluated by protein adsorption experiment. The analytical results show that the imprinted poly：ner has good selectivity to bovine serum albumih. The bovine serum albumin adsorption of the molecular imprinting polymer is 21.9 times of the non-imprinted polymer. The imprinted polymer shows good selective adsorption and separation ability to the bovine serum albumin.