吉西他滨联合维生素C、维生素K_3应用于人膀胱癌T24系的实验研究

目的观察吉西他滨联合维生素C和维生素K3对体外培养的人膀胱癌T24细胞系的作用,探讨VC+VK3对吉西他滨诱导细胞凋亡的影响。方法采用体外培养技术,MTT法观察细胞抑制作用,分别筛选出吉西他滨、VC+VK3的最佳作用浓度。流式细胞术检测药物作用的细胞凋亡和细胞周期分布。结果药物作用72h后,吉西他滨联合VC+VK3组凋亡率为(41.39±1.04)%,吉西他滨组为(9.23±1.08)%,VC+VK3组为(17.42±1.09)%,空白对照组为(5.92±0.76)%,各组间差异具有显著性(P<0.05)。与对照组相比,联合用药组细胞周期分布出现S期上升。结论吉西他滨联合VC+VK3协同作用于人膀胱癌T24系细胞,VC+VK3增强吉西他滨诱导细胞凋亡的作用。

Objective To evaluate the effect of gemcitabine plus vitamin C and vitamin Ka on human bladder cancer cell line T-24 and investigate the influence of vitamin C and vitamin K3 on the gemcitabine-induced apoptosis. Methods By cells culturing in vitro, MTT is used to assess the anti- proliferation effects and select the best concentrations of gemcitabine and VC＋VK3 separately.Apoptosis rate and cell cycle distribution were detected by the flow cytometry. Results There were （41.39±1.04）% apoptosis with gemcitabine plus VC＋VK3, （9.23±1.08）% apoptosis with gemcitabine alone,（17.42± 1.09）% apoptosis with VC＋VK3 alone and （5.92±0.76）% apoptosis with control group. The effect of gemcitabine plus VC＋VK3 showed a cell cycle specificty, blocking at S phase compared with control group. Conclusion Gemcitabine plus VC＋VK3 have significant antiproliferative and apoptotic effects.VC＋VK3 enhances the effects of gemcitabine-induced apoptosis against bladder cancer.