参附注射液对重症急性胰腺炎大鼠NO和ET-1的影响

Effects of Shenfu injection on nitric oxide and endothelin-1 to the rats with severe acute pancreatitis

目的本研究通过对参附注射液在SD大鼠重症急性胰腺炎模型上的使用,观察其对血清及胰腺组织NO和ET-1的影响,探讨其作用机理,为临床使用提供实验依据。方法48只SD大鼠随机分为3组:假手术组(n=16)、SAP组(n=16)、SF组(n=16)。假手术组开腹后仅翻动肠管,SAP组、SF组以3%牛磺胆酸钠2mL/kg逆行注入胆胰管,复制重症胰腺炎模型。制模成功后,SF组大鼠,尾静脉注射参附注射液,假手术组、SAP组给予等量生理盐水,每12h1次。各组分别于制模后12h、24h两个时间点取材。观测腹水,检测血清淀粉酶及血清和胰腺组织NO、ET-1浓度,并计算ET-1/NO比值,胰腺组织行HE染色光镜检查形态学变化。结果各时点血清淀粉酶活性、胰腺病理评分:SAP组、SF组显著高于假手术组(P<0.01),SF组低于SAP组(P<0.01);血清、胰腺组织内ET-1、NO浓度:SAP组、SF组显著高于假手术组(P<0.01),SF组显著低于SAP组(P<0.01);血清、胰腺组织内ET-1/NO比值,SAP组显著高于假手术组(P<0.01),SF组与SAP组相比降低(P<0.05,P<0.01)。结论SF组与模型组相比较,血清、胰腺组织NO、ET-1浓度下降差异有统计学意义,与此同时血清淀粉酶活性,胰腺湿干重比值降低,表明参附注射液可减轻胰腺组织损害,对胰腺组织有保护作用。

Objective To observe the effects on nitric oxide （NO） and endothelin-1 （ET-1） in ser um and pancreatic tissue, and search for the mechanism and provide the laboratory basic for the clinic application by using the Shenfu （SF） injection to model SD rats with severe acute pancreatitis. Methotis 48 SD rats were divided randomly into 3 groups which were Shum operated group （n= 16）, SAP group （n=16）, and Shenfu injection （SF） treatment group （n=16）. The rats are killed at 12h and 24h respectively after operation, blood and tissue samples are collected to detect the indexes as follows： Serum amylase NO and ET-1 levels in blood serum and pancreatic tissue. Pancreatic tissue HE dyeing and light microscope detection of pancreatic tissue. Results SAP was induced in SD rats by the retrograde injection of 3% sodium taurocholate （2 mL/kg） into the biliopanereatic duct. Blood serum amylase levels and pancreatic histologic scores were significantly reduced in SF group rats given SF compared with those in SAP group respectively （P〈0.01, P〈0.05）. NO and ET-1 concentration in serum and tissue were increased in group SAP and SF rats compared with those in Shum operated group （P〈0.01）, nitric oxide and ET-1 concentration in SF group were decreased against those in SAP group （P〈0.01）. ET-1/NO ratio in serum and tissue were increased in SAP group compared with those in Shum operated group（P〈0.01）, ET-1/NO ratio in serum and tissue were decreased in SF group compared with those in SAP group （P〈0.05 ,P〈0.01 ）. Conclusion Treatment with SF appears to have ameliorated the course of SAP.