不同顶体功能精子优选后受精结局的研究

The result of fecundation after different sperm acrosin gradient-density optimization

目的比较顶体酶活性正常及异常的精子通过密度-梯度优选后,用于体外受精(IVF)的受精率及优质胚胎率的差异,了解顶体酶检查在IVF中的意义。方法对94例不育男性精液标本进行顶体酶活性检测后,分为顶体酶正常组和异常组。密度-梯度优选后分别用于IVF授精,比较两组受精率及优质胚胎率。结果两组精子授精后,受精率及优质胚胎率差异无明显统计学意义(P>0.1)。结论精子顶体酶活性检查指导临床意义不明确,顶体酶活性异常的精子,经密度-梯度法优选后可获与顶体酶活性正常精子一致的治疗效果。

Objective To understand the significance of sperm acrosin assay in vitro fertilization （IVF） by the differences comparison of fertilization rate and good embryos rate which were two groups of sperm samples with normal and abnormal acrosin activity were used IVF after gradient-density opti mization. Methods Semen samples from 94 infertile men after sperm acrosin assay were divided into two groups which were normal activity group and abnormal activity group. The fertilization rate and good embryo rate were compared through using IVF after gradient-density optimization. Results The results showed that there were no statistically significant differences between fertilization rate and good embryo rate comparing with two groups （P〉0. 1）. Conclusion The clinical value of sperm acrosin assay is unclear. Sperm with abnormal acrosin activity could obtain the similar efficacy compared with sperm with normal acrosin activity by using gradient-density centrifugal technique.