摘要
The intra-population phylogenetic relationship between Micropterus salmoides and Tilapia nilotica was analyzed by random amplified polymorphic DNA （ RAPD） technique. The results showed that the similar coefficient of bands between M. salmoides and T. nilotica was 0.236, and the genetic distance was 0. 764, which indicated that M. salmoides had a distant relationship with T. nilotica.
利用随机扩增多态性DNA(RAPD)技术对加洲鲈和罗非鱼种群间的亲缘关系进行分析。品种间带纹相似系数(共享度)根据Lynch的公式进行计算:F_(xy)=2N_(xy)/N_x+N_y(N_(xy)是RAPD指纹图谱中品种X和品种Y的共有带纹数,N_x和N_y分别为品种X和品种Y各自可分辨带纹总数)。品种间的遗传距离(D)根据2个品种的共享度(F)来计算:D=1-F。根据遗传距离的D值,再用UPGMA法求出各类间距离后再聚类。结果显示:从20个随机引物中筛选出4个清晰,多态性丰富的引物用于分析。各种群平均每一引物所获得的带纹数为:加洲鲈7.8±2.45,罗非鱼5.1±1.87。4个引物共扩增出加洲鲈35条带其中特异性带16条,罗非鱼21条带其中特异性带8条,测得加洲鲈与罗非鱼之间的带纹相似系数为0.236,遗传距离为0.764,表明加洲鲈和罗非鱼之间的亲缘关系较远。
