RGD肽修饰的纯钛种植材料表面成骨细胞黏附增殖能力

A study of the osteoblasts adhesion,growth and proliferation on the surface of pure titanium immobilized by RGD peptide

目的:评价精氨酸-甘氨酸-天冬氨酸(RGD)肽修饰纯钛表面对细胞早期黏附增殖影响。方法:利用分子自组装技术氨基化纯钛表面,化学接枝GYRGDS肽,计算不同时点材料表面黏附细胞数;不同浓度RGD肽液预处理接种前细胞,计算材料表面细胞黏附率;MTT法和扫描电镜分别检测材料表面细胞的增殖、生长。结果:RGD肽修饰的纯钛表面细胞早期黏附率、增殖活性和生长情况在各时点均优于未修饰组,高浓度肽液预处理对RGD肽修饰组表面细胞有较强黏附抑制作用。结论:采用分子自组装技术可将活性RGD肽稳定的化学接枝于纯钛表面,修饰后材料的细胞早期黏附增殖等生物学行为有明显改善。

Objective：To evaluate the effect of pure titanium modified by bioadhesive RGD peptide on the early attachment,growth and proliferation of osteoblasts.Methods：The titanium samples were hydroxylated by alkali/hot water aging and sol-gel layer-by-layer deposition technique.Afterwards,the terminal-NH2 group was introduced to the titanium surface by organosilane APTMS self-assembled monolayers and the functional group-NH2 was further reacted with EDC/NHS by which RGD peptides was covalently immobilized to titanium.The efficiency of this bioreactive surface in promoting cell attachment and the competitive inhibition effect of RGD peptide with different concentrations were observed by calculating the amount of osteoblasts attached on the modified titanium.The growth and proliferation were observed by MTT method and scanning electronic microscopy.Results：The cell adhesion percentage of the RGD modified titanium group was much higher than that of the other groups.The RGD peptide solutions with higher concentration had stronger inhibitory impact on the cell adhesion onto the titanium surface.The cell growth,morphology and proliferation on the RGD peptide modified titanium were better than other groups.Conclusion：Bioadhesive peptide can be chemically grafted onto the titanium surface by means of self-assembled monolayers technique.The cells′ biological behaviors on the surface of RGD immobilized titanium are greatly improved in vitro.