百脉根侧部花瓣发育突变体的研究(英文)

Mutations Affecting Lateral Petal Development in Lotus japonicus

为了研究调控花瓣形状发育的基因网络,我们在豆科模式植物百脉根中筛选影响侧部花瓣发育的突变体。我们鉴定了两个独立的突变体:kew2和kew3。这两个突变体有相似的表型:突变体的侧部花瓣转化为类似野生型的腹部花瓣。遗传分析表明kew3和已报道的kew1等位,由同一个单隐性基因位点控制。kew1突变体定位于基因座位KEW1,与豌豆K位点直系同源。kew2的遗传分析暗示在侧部花瓣发育中有其它遗传因子与kew有相互作用。对这些突变体的分析和基因克隆有助于阐明两侧对称花型发育的调控机制和理解两侧对称花型的进化途径。

To investigate the genetic loci to regulate the development of petal shape, screening of the mutation affecting lateral petal development was conducted in a model plant of legume, Lotus japonicus. Two independent mutants, kew2 and kew3 with the similar phenotype were obtained, whose shape of lateral petal is altered and resembles the one of ventral petal in the wild type. Genetic analysis showed that kew3 was caused by the same single recessive locus and allelic to the one of kewl, a formal identified mutant in the locus KEW1. KEW1 has been shown to be an ortholog of K in pea, but whose mutation at the molecular level is unsolved yet. Furthermore, genetic analyses of kew2 indicate that there are other genetic factors which could interact with kew and involve in the lateral petal development. Analysis of these mutations and cloning the corresponding loci will shield light on the underlying molecular mechanism in the control of floral asymmetry and facilitate our understanding on the evolution of the zygomorphic development.