应用Alamar Blue法与试管法测定α-倒捻子素和8-甲氧基补骨脂素体外抗结核杆菌活性

Application of Alamar Blue and two folds dilutions methods for determining in vitro antimycobacterial activity of α-mangostin and 8-methoxypsoralen

选取传统中药提取单体化合物α-倒捻子素和8-甲氧基补骨脂素,通过2种药物敏感性实验方法MABA(Microdilution Alamar Blue Assay)分析与试管法分别测定了两种化合物对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)的体外抗菌活性,同时还测定了9种阳性抗结核药物对这两种结核菌的最低抑菌浓度(MIC),比较2种方法的检测结果。结果表明:α-倒捻子素对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)的最低抑菌浓度均为6.25μg/mL,而8-甲氧基补骨脂素对结核分枝杆菌H37Rv(ATCC27294)和H37Ra(ATCC25177)的最低抑菌浓度均为128μg/mL。Alamar B1ue法与试管法相比较,完全符合率是90%(9/11),具有较好的一致性。本研究结果表明Mamar B1ue法是一种快速、简便测定药物对结核分枝杆菌MIC的方法。本研究为α-倒捻子素和8-甲氧基补骨脂素的进一步开发利用和抗分枝杆菌机制研究打下了基础。

α-mangostin and 8-methoxypsoralen （8-MOP） are active constituents from typical traditional Chinese medicine Garcinia mangostana L.and Psoralea corylifolia L., respectively. The antimycobacterial susceptibility ofα -mangostin and 8-MOP were determined by the microdilution alamar blue Assay （MABA） and the conventional two folds dilutions method against Mycobacterium tuberculosis （M. tuberculosis） H37Rv （ATCC 27294） and M. tuberculosis H37Ra （ATCC 25177）, α-mangostin showed a minimal inhibitory concentration （MIC） of 6.25 μg/mL against M. tuberculosis H37Rv and M. tuberculosis H37Ra, and 8-MOP showed a minimal inhibitory concentration （MIC） of 128 μg/mL against M. tuberculosis H37Rv and M. tuberculosis H37Ra. These results will pave the way to further development and exploring the mechanism of α-mangostin and 8-MOP against mycobacterium.