光谱法研究芹菜素与牛血清白蛋白的相互作用

Study on the Interaction of Bovine Serum Albumin with Apigenin by Spectral Methods

在模拟人体的生理条件下(pH=7.40,Tris-HCl),采用荧光光谱研究了牛血清白蛋白与芹菜素的相互作用。研究结果表明牛血清白蛋白与较小浓度的芹菜素间的相互作用属于静态猝灭过程;随着芹菜素浓度的增加,与牛血清白蛋白间的相互作用为静态猝灭和动态猝灭共同作用,但仍以静态猝灭为主。进一步求出了在287K和310K温度条件下静态猝灭的猝灭常数,由求得的热力学参数,证明了芹菜素与牛血清白蛋白之间主要依靠静电引力结合,采用同步荧光光谱技术探讨了芹菜素对牛血清白蛋白构象的影响。

The interaction of BSA and apigenin was investigated by absorption spectra and fluorescence spectroscopy. The static quenching exits between apigenin and BSA in physiological solution in lower apigenin concentration, but the static quenching and dynamic quenching exits together between apigenin and BSA in physiological solution in the higher apigenin concentration. The quenching constant is obtained at different temperatures （287K, 310K）. According to the thermodynamic parameters,it shows that binding power between apigenin and BSA is mainly by static electricity force,synchronous spectrum is used to investigate the conformational changes of BSA.