紫外光分光光度法测定淫羊藿中维生素C的含量

Determination of Vitamin C in Herba Epimedii by Ultraviolet Spectrophotometry

紫外光分光光度法测定淫羊藿中维生素C含量。测定波长为243nm;维生素C浸提剂为1.0%(V/V)盐酸溶液;维生素C浓度在2—50mg/L范围内与吸光度线性关系良好(r=0.9992,n=3);回收率在97%—115%之间(n=3);相对标准偏差都小于6.0%(n=3);检出限(LOD)和定量限(LOQ)分别为1.33mg/L和4.04mg/L。人工栽培的箭叶淫羊藿、天平山淫羊藿和长蕊淫羊藿鲜叶中维生素C的含量不同。

Vitamin C in herba epimedii was determined by ultraviolet spectrophoton ctry at 243nm;1.0% （V/V） HCl aqueous solution was selected to extract vitamin C from fresh Epirnedium leaves. Calibration curve was found in the range of 2--50mg/L and the correlation coefficient of linear regression analysis was 0. 9992（n=3）. Recovery of vitamin C was between 97% and 115%（n=3）. Relative standard deviations（RSDs）were less than 6.0% （n=3）. LOD and LOQ were 1.33mg/L and 4. 04ng/L, respectively. A variation of the contents of vitamin C was found among the cultivated samples of E. sagittatum , E. rnyrianthum and E. dolichosternon.