抑肽酶对大鼠局灶性脑缺血再灌注后白细胞介素-1β、白细胞介素-6和自细胞介素-8的影响

Effects of Aprotinin on the levels of cerebral IL-1β, IL-6 and IL-8 in rats experienced focal cerebral ischemia reperfusion

目的观察抑肽酶对大鼠局灶性脑缺血再灌注后脑损伤的保护作用。方法复制大鼠大脑中动脉栓塞模型（MCAO／R），采用ELISA法测定大鼠脑组织内IL-1β、IL-6和IL-8的含量，并进行神经功能评分。结果模型组IL-1β、IL-6和IL-8蛋白含量在3h即有增加，12h达高峰，而抑肽酶组相应各时间点的蛋白含量表达明显减少；模型组与抑肽酶组在3h的神经评分差异无统计学意义，其余各时间点均差异有统计学意义。结论抑肽酶对大鼠脑缺血再灌注损伤有一定保护作用，其作用机制可能与降低脑组织中IL-1β、IL-6和IL-8含量有关。

Objective To observe the protective effect of aprotinin on the cerebral ischemia-reperfusion injury. Methods Rat model of cerebral ischemia-reperfusion injury was created by the middle cerebral artery embolization. The changes of IL-1β, IL-6 and IL-8 content in the brain tissue were measured by ELISA. The neurological scores were made on Zea Longa 5-point scale. Results In model group,the IL-1β,IL-6 and IL-8 protein content began to increase slightly at 3 hours compared with sham group, reached the peak at 12 hours. In the aprotinin treated group, aprotinin reduced the protein content. It showed no significant difference between model group and aprotinin treated group at 3 hours, however and there was greatly difference at 6,12 and 24 hours between these two groups. Conclusion The present study provides in vivo evidence that aprotinin protects brain against cerebral ischemia-reperfusion injury. The mechanism is related to the decrease of cerebral levels of IL-1β ,IL-6 and IL-8.