促红细胞生成素对大鼠急性心肌梗死心肌细胞Bcl-2、Bax表达的影响

Effects of erythropoietin on expressions of myocardial cell Bcl-2 and Bax in rats with acute myocardial infarction

目的探讨促红细胞生成素（EPO）对大鼠急性心肌梗死（AMI）的心肌细胞Bcl-2、Bax表达影响。方法将27只大鼠制作AMI模型后随机分为：治疗组16只和对照组11只。采用腹腔注射方法，治疗组给予EPO3000U／kg，共3d（术前1d手术、当天和术后1d），对照组在相同的时间点给予等量的0．9％氯化钠注射液。6周后，采用导管法测定左室有创血流动力学指标；免疫组化法检测Bcl-2、Bax表达水平。结果治疗组的LVSP和＋dp／dtmax较对照组明显升高（P〈0．05），LVEDP和-dp／dtmax较对照组明显降低（P〈0．05）；两组心率变化不大（P〉0．05）。治疗组的心肌细胞Bcl-2的表达明显高于对照组（P〈0．05）；心肌细胞Bax的表达及Bcl-2／Bax明显低于对照组（P〈0．05）。结论EPO对缺血和梗死心功能的保护作用可能与心肌细胞凋亡的减少有关。

Objective To study the effects of erythropoietin on expressions of myocardial cell Bcl-2 and Bax in rats with acute myocardial infarction（AMI）. Methods Rats that survived after AMI were randomly divided into treatment group and control group. Treatment group were given EPO 3000 U/kg, A total of 3 d （preoperative 1 day, the same day,after 1 day） ,control group at the same point in time given the same amount of saline by the intraperitonealy injection. After 4 weeks, hemodynamics and left ventricular function were measured with catheterization, Bcl-2 and Bax were analyzed by immunohistochemical method. Results After 4 weeks, LVSP and ＋ dp/dtmax in treatment group were significantly higher than those in control group（ P 〈 0. 05 ）, LVEDP and - dp/dtmax were significantly lower than those in control group（P 〈 0. 05 ） ;Change of heart rate in two groups were no difference（P 〉 0. 05）. The expression of myocardial cell Bcl-2 in treatment group were significantly higher than that in control group（ P 〈0. 05 ） ;the expression of myocardial cell Bax and Bcl-2/Bax in treatment group were significantly lower than that in control group （ P 〈 0. 05）. Conclusion The protective effect of EPO on ischemia and infarction of cardiac function may be related to the reduction of cardiomyocyte apoptosis.