膀胱移行细胞癌中Survivin蛋白和PTEN蛋白的表达及意义

Expression and clinical significance of survivin and PTEN in bladder transitional cell carcinoma

目的探讨Survivin蛋白与PTEN蛋白在膀胱移行细胞癌中的表达和意义。方法用免疫组织化学S-P法检测62例膀胱移行细胞癌组织及10例正常膀胱黏膜组织中Survivin蛋白和PTEN蛋白的表达。结果10例正常膀胱黏膜组织中PTEN蛋白表达均为阳性，Survivin蛋白表达均为阴性；PTEN在膀胱移行细胞癌中阳性表达率为46．8％（29／62），肿瘤不同分级中随恶性程度的增高，表达减少，Ⅰ级与Ⅲ级比较，差异有统计学意义（P〈0．05），不同临床分期中随分期的增高，表达减少，Tis—T1与T2～T4比较，差异无统计学意义（P〉0．05）；Survivin蛋白在膀胱移行细胞癌中阳性表达率为56．5％（35／62），肿瘤不同分级中随恶性程度的增高，表达增高（P〈0．05），不同临床分期中随分期的增高，表达增高，差异有统计学意义（P〈0．05）；PTEN蛋白和Survivin蛋白表达负相关（P〈0．05）。结论Survivin蛋白和PTEN蛋白异常表达可作为膀胱移行细胞癌肿瘤标志物。

Objective To investigate the expression and chnical significance of survivin and PTEN in bladder transitional cell carcinoma（TCC）. Methods The expression of survivin and PTEN was studied by S-P immunohistochemisty in 10 cases of normal urinary bladder tissues and 62 cases of the bladder TCC. Results All the normal urinary bladder tissues showed positive staining pattern of PTEN protein, negative staining pattern of survivin protein, positive rate of PTEN in 62 eases of the bladder TCC is 46. 8% ,the difference of positive rate between grade Ⅰ and grade Ⅲ was significant （ P 〈 0. 05）, the difference of positive rate between Tis Tis- T1 and T2-T4 showed a trend of decreasing,but there was no statistically significant difference （ P 〉 0. 05 ） ; positive rate of survivin in 62 cases of the bladder TCC is 56.5% ,the difference of positive rate between grade Ⅰ and grade Ⅲ was significant（P 〈0. 05） ,the difference of positive rate between Tis - T1 and T2 - T4 was significant too（ P 〈 0. 05 ）. The expression of PTEN protein and survivin protein was reversely correlated（ P 〈 0. 05）. Conclusion The abnormal expression of PTEN protein and survivin protein play an important role in the occurrence and progress of the bladder TCC. It can be regarded as a useful diagnostic marker in the bladder TCC.