兔深低温停循环体外循环模型的建立

A Rabbit Model of Cardiopulmonary Bypass under Deep Hypothermia Circulatory Arrest

目的建立新西兰兔深低温停循环（deep hypothermic circulatory arrest,DHCA）体外循环（cardiopulmonary bypass,CPB）模型。方法健康新西兰兔10只,雌雄不限,体重（2.5±0.5）kg。动脉套管针穿刺连接主动脉灌注管,右心耳切开连接静脉引流管建立体外循环。肛温降至20℃时停循环,维持兔直肠温度16℃-20℃,持续60 min后,恢复循环并复温至36℃时脱离体外循环。监测心电图、血液动力学变化。结果9只成功建立深低温停循环体外循环模型,1只死于模型建立过程中出血。预充溶液75 mL,平均转流时间140 min,CPB过程中平均动脉压在正常范围内,9只兔主动脉开放后心脏成功复跳。结论新西兰兔深低温停循环体外循环模型稳定、可行,成功率较高,是进行低温停循环体外循环基础研究、脑保护作用及其机制研究的理想模型。

Objective To establish the model of cardiopulmonary bypass （CPB） under deep hypothermia circulatory arrest （DHCA） in the rabbits. Methods 10 health rabbits weighting 2.5 ± 0.5 kg were used in this study. A 16-gange tmcar was inserted directly as an aortic eannula, and a metallic plug of the venous eannula was inserted into the right aurieular appendage to drain venous blood. Deep hypothermic circulatory arrest lasted for 60 minutes. After systemic eooling and the establishment of a hypothermie rectal temperature of 20 ℃, DHCA was induced for 60 minutes. The animals were then reperfused and rewarmed to achieve a normal rectal temperature of 36℃. Electrocardiogram and hemodynamics were monitored in the total procedure. Results The model of CPB under DHCA was successfully established in 9 rabbits （one rabbit died of bleeding in the surgery）. The primary volume of fluid was 75 mL, and the CPB circuit was 140 minutes on average. Mean arterial pressure was within normal limits. Conclusion Rabbit model of CPB under DHCA was feasible and stable to research pathophysiological and pathological changes during CPB and the effect and mechanism of protection of cerebrum function.