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竞争抑制ELISA法检测植物中豌豆胰岛素PA1b 被引量：1

Quantitative Analysis of Pea Albumin 1b (PA1b) in Several Plants by Competitive Inhibiting ELISA
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摘要 为研究豌豆胰岛素PA1b在植物生长发育中的作用,利用竞争抑制ELISA法,检测PA1b在豌豆、芋头、姜、西红柿、苦瓜、蒜头、洋葱、丝瓜、黄瓜等植物中的分布,以及PA1b在豌豆种子发芽过程中的含量变化。结果显示PA1b在豌豆中含量最高,且随着豌豆种子发芽时间的增加含量逐渐减少,PA1b可能对植物生长发育起调控作用。 The concentration of PAlb in several plants including pea, taro, ginger, tomato, bitter gourd,garlic,onion, towel gourd and cucumbers was measured by competitive inhibiting enzyme-linked immunosorbent assay （ELISA） method to study the role of pea albumin lb （PAlb） during the development of plants. The results show that the pea contains much more PAlb than other plants do, and the PAlb content reduces with the germination time.
作者 赵燕英 王建和 顿新鹏 陈正望 陆婕
机构地区 华中科技大学生命科学与技术学院
出处 《华中农业大学学报》 CAS CSCD 北大核心 2010年第1期111-113,共3页 Journal of Huazhong Agricultural University
基金 国家"863"高技术研究发展计划(2002AA214061) 国家自然科学基金项目(30370647 30470823)资助
关键词 豌豆胰岛素PA1b 竞争抑制ELISA法 发芽 PAlb ELISA germination
分类号 Q503 [生物学—生物化学]
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参考文献12

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华中农业大学学报
2010年 第1期

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