微卫星不稳定性与卵巢癌的初步研究

Study for microsatellite instability of ovarian cancer

目的检测微卫星不稳定与卵巢癌的关系。方法收集卵巢癌患者及对照组的血液标本,提取循环血DNA,设计特异性引物,采用PCR-SSCP及测序分析的方法检测卵巢癌患者循环血中微卫星不稳定性与卵巢癌易感性的关系,分析微卫星不稳定性与卵巢癌患者的病理类型、临床病理分期等相关性。结果卵巢癌组中浆液性囊腺癌、粘液性囊腺癌、子宫内膜样癌、库肯勃瘤及其他类型发生微卫星不稳定的频率相比较差异具有显著性(P<0.05)。微卫星不稳定在早期和晚期中相比较差异具有显著性(χ2=18.59,P<0.05)。91例卵巢癌患者中发生微卫星不稳定的频率为47.25(43/91),良性肿瘤和正常人血中未检测到微卫星不稳定性的发生。结论卵巢癌患者外周静脉血循环DNA中存在微卫星不稳定性;微卫星不稳定与卵巢癌病理类型、临床病理分期均有关;微卫星不稳定主要发生在卵巢恶性肿瘤中,良性肿瘤及正常人中不存在微卫星不稳定。

Objective To detect the relationship between microsatellite instability and ovarian cancer. Methods First, collect the blood samples of ovarian cancer patients and control group, extract the circulating DNA, designed primers, using the method of PCR-SSCP, sequancing to detect microsatellite instability and susceptibility in circulating blood of ovarian cancer patients, analysis the relationship between microsatellite instability in ovarian cancer patients with histological types, clinical staging and other related respects. Results microsatellite instability in serous adenocarcinoma, mucinous cystadenocarcinoma, endometrioid carcinoma, Krukenberg tumor and other types the difference was significant （ P 〈 0.05） . Microsatellite instability in early and late stage the difference was significant（χ^2 = 18.59, P 〈 0.05）. 91 cases of ovarian cancer patients with microsatellite instability occm, s in the frequency of 47.25 （43/91）. benign tumor and normal blood did not detect the occurrence of microsatellite instability. Conclusion The microsatellite instability exist in the circulating DNA of the ovarian cancer patients; Microsatellite instability and pathological types of ovarian cancer, clinical staging were related; Microsatellite instability occurs mainly in ovarian cancer,does not exist in the benign tumors and normal Microsatellite instability.