衍生化分光光度法测定依诺沙星和诺氟沙星

Determination of Enoxacin and Norfloxacin by Spectrophotometry with Derivation

碱性介质中，依诺沙星和诺氟沙星均能与1，2-萘醌-4-磺酸钠（NQS）发生反应而生成橙红色的物质，并使最大吸收波长发生大幅度的红移。据此建立了测定这两种药物的分光光度新方法。依诺沙星和诺氟沙星的浓度分别在0．8～16mg·L^-1和0．9～18mg·L^-1范围内符合比耳定律；衍生物在454nm和462nm波长处的表观摩尔吸光系数分别为1．86×10^4L·mol^-1·cm^-1和1．93×10^4L·mol^-1·cm^-1；两种药物的检出限分别为0．13mg·L^-1和0．19mg·L^-1。该方法已成功用于药物制剂中依诺沙星和诺氟沙星含量的测定。其回收率为96．63％～99．55％，相对标准偏差为0．56％～0．89％．

A method has been established for the determination of enoxacin and norfloxacin using sodium 1,2-naphthoquinone-4-sulfonate as the chemical derivative chromogenic reagent, This method is based on the formation of salmon pink compounds from the nucleophilic substitution reactions of enoxacin and norfloxacin with sodium 1,2-naphthoquinone-4-sulfonate in alkali buffer solution and a red-shift of the maximum absorption wavelength was concentration range of 0. 8- 16 mg^-1 observed. The linear calibration 1 for enoxacin and 0. 9-18 mg·L^-1 graphs were obtained in the for norfloxacin. The apparent molar absorptivity of the complex at 454 and 462 nm are 1. 86×10^4 and 1.93×10^4 L·mol^-1·cm^-1, and the detection limits are 0. 13 and 0. 19 mg·L^-1, respectively. The proposed method has been successfully applied to the determination of the investigated drugs in their pharmaceutical preparations with recoveries ranging from 96.63% to 99.55% and RSDs from 0.56% to 0.89%.