三草方提取物对肺腺癌A549细胞的作用

Effect of Sancao prescription extracts on lung adencarcinoma cell line(A549)

目的:研究三草方(夏枯草、白花蛇舌草、仙鹤草)的不同极性提取部位及其配伍后对人肺腺癌A549细胞株增殖活性的影响。方法:采用醇提取法和水提取法分别制备三草方的95%、60%、30%醇提部位、水提部位;以MTT法测定水煎液和各不同极性部位及其配伍后对A549细胞的增殖活性的影响。结果:在三草方60%醇提部位对A549细胞的IC50最低;60%和95%醇提部位配伍后有明显的协同抑瘤活性。结论:三草方的95%和60%醇提部位间的配伍具有良好的抗肺癌作用。

AIM： To investigate the ethanolic and aqueous extracts from Sancao prescription （Spica prunellae, Oldenlandia diffuse （willd） Roxb, Herba agrimoniae） on the proliferation of human lung adenoearcinoma cell line （A549）. METHODS： 95% ,60% and 30% ethanolie extract and aqueous extract were prepared from Sancao pre- scription. The MTF assay was used to determine the inhibitory action against the proliferation of A549. RESULTS ： IC50 of 60% ethanolic extract over A549 was one of the lowest in extracts. Combination of 60% and 90% ethanolic extract showed the synergistic antitumour activity. CONCLUSION： Ethanolic extract of Sancao prescription has and effect on human hung adenocareinoma（A549）.