当归流浸膏质量标准的研究

Quailty standards for Chinese extractum angelicae liquidum

目的:建立当归流浸膏的质量控制标准。方法:对当归流浸膏中阿魏酸和藁本内酯采用薄层色谱法进行定性鉴别,采用HPLC梯度洗脱法进行定量检测,色谱条件为Diamonsil ODS C18柱(250 mm×4.6 mm,5μm),流动相A-甲醇(含0.4%冰醋酸),B-0.4%冰醋酸水溶液,梯度洗脱(0～15 min,38%A;15～20 min,38%～70%A;20～40 min,70%A;40～45 min,70%～38%A),检测波长为323 nm,流速为1.0 mL/min,柱温为35℃。结果:阿魏酸和藁本内酯分别在1.008～10.08μg/mL和9.985～99.85μg/mL范围内线性关系良好,平均回收率分别为98.11%和101.61%,RSD值分别为1.58%和1.32%。结论:该方法简便易行,具有良好的重现性和稳定性,可用于当归流浸膏的质量控制。

AIM： To establish the quality standards for Chinese extractum angelicae liquidum. METHODS： TLC was used to identify ferulic acid and ligustilide and HPLC to determine the content of ferulic acid and ligustilide. HPLC was performed on a Diamonsil ODS-C18 analytical column （250 ×4.6mm,5μm） with gradient elution（0-15 min, 38%A; 15-20 min, 38%A-70%A; 20-40 rain, 70%A; 40-45 min, 70%A-38%A） of methanol （ A, containing 0.4% glacial acetic） and 0.4% glacial acetic acid（B） at the flow rate of 1.0 mL/min. The diode array detection wavelength was set at 323 nm and the column temperature was at 35 ℃. RESULTS： The linear range of ferulic acid and ligustilide were from 1. 008 μg/mL to 10.08 μg/mL and 9. 985 μg/mL to 99.85 μg/mL, the average recoveries of both were 98.11% and 101.61%, RSD were 1.58% and 1.32%. CONCLUSION： The method is rapid, simple and accurate with high reproducibility and can be used to control the quality of Chinese extractum angelicae liquidum.