HPLC测定安神养血口服液中3种生物碱延胡索乙素、钩藤碱和异钩藤碱含量

Determination of tetrahydropalmatine and rhynchophylline and isorhynchophylline in Anshen Yangxue Oral Liquid by HPLC

目的:深入研究安神养血口服液(钩藤、金不换、制何首乌、首乌藤、松针)含量测定方法,为该药质量标准研究和完善提供科学依据。方法:HPLC测定该药中3种生物碱延胡索乙素、钩藤碱和异钩藤碱含量,色谱柱:依利特Kromasil C18柱(5μm,4.6 mm×250 mm),流动相:甲醇-水(55∶45)(含0.01 mol/L三乙胺,用冰醋酸调pH值至7.5),柱温:25℃,15 min前检测波长为281 nm,15 min后为254 nm,流速1.0 mL/min。结果:延胡索乙素、钩藤碱、异钩藤碱平均回收率分别为98.47%,99.04%,98.75%,延胡索乙素在进样量0.01μg～0.10μg范围内、钩藤碱在进样量0.16μg～1.6μg范围内,异钩藤碱在进样量0.12μg～1.2μg范围内与峰面积呈良好线性关系。结论:该方法简便、快速和准确,可用于该药质量控制。

AIM ： To establish the method for determining tetrahydropalmatine ,rhynchophylline and isorhynchophylline in AnshenYangxue Oral Liquid （Ramulus uncariae cum urtcis, stephania kwangsiansis, Radix polygoni multiflori praeparata cum succo glycinus sotae, Caulis Polygoni multiflori and Pine needle）. METHODS ： Tetrahydropalmatine,rhynehophylline and isorhynchophylline were determined by HPLC. Chromatographic condition was composed of Kromasil C18 column, a mixture of methanol and water（55：45 ） as mobile phase with 0.01 mol/L triehthylamine, adjusted with acetic acid to pH of 7.5, UV detection wavelength of rhynchophylline and isorhynchophylline was set at 254 nm, UV detection wavelength of tetrahydropalmatine was set at 281 nm. RESULTS ： The averagere recoveries of tetrahydropalmatine, rhynchophylline and isorhynchophylline were 98.47% ,99.04% and 98. 75% respectively; RSD were 0.95% ,2.6% and 1.6%, respectively. CONCLUSION： This method is simple, sensitive,accurate, and can be used for determining tetrahydropalmatine,rhynchophylline and isorhynchophylline in Anshen Yangxue Oral Liquid.