家兔微血栓栓塞中风模型的建立及应用

Establishment and Application of Microthrombus Embolic Stroke Model in Rabbit

目的研究脑血栓模型的建立及应用。方法采用微血栓经颈内动脉栓塞兔大脑中动脉的方法,建立微血栓栓塞中风模型并观察应用巴曲酶的效果。结果微栓子量达到(0.63±0.57)mg时可使一半动物出现中风症状,模型科学、可靠。巴曲酶0.5BU/kg治疗后6h能明显提高致半数卒中微栓子量(ES50),1.0BU/kg作用更明显,治疗后6h及24hES50分别提高了2.2倍、1.8倍;巴曲酶1.0BU/kg给药后1h可使组织型纤溶酶原激活剂(t-PA)含量明显增加,较模型对照组增加了23.25%;巴曲酶0.5BU/kg及1.0BU/kg给药后可使纤维蛋白原(Fbg)含量明显降低,以给药后6h最明显;巴曲酶0.5BU/kg及1.0BU/kg给药后对脑出血类型及出血率无明显影响。结论采用微血栓经颈内动脉栓塞大脑中动脉形成微血栓栓塞中风模型,方法成功,药物评价结果可靠。

Objective To establish the microthrombus embolic stroke model and to study the effect of batroxobin in this model. Methods The embolie stroke model in 16 rabbits were established with embolization by injecting the suspension of micro blood clots into rabbit middle cerebral artery（MCA） via carotid catheter. At 5min after embolization, batroxobin（0.5, 1.0 BU/kg） was infused intravenously for 5 min. Behavioral analysis was conducted at 6, 24 h after embolization. Assessment of t-PA, fibrinogen （Fbg） and hemorrhage was concluded in this study. Results When the amount of micro blood clots reached to （0.63±0. 57）rag, the stroke symptoms occurred in 50% of the rabbits. The model was scientific and reliable. ESso was obviously increased after 6 h of batroxobin treatment in a dose of 0. 5 BU/kg. The effect was more obvious in a dose of 1.0 BU/kg. ES50 was increased by 2.2, 1.8 times after 6, 24 h of treatment, respectively, t - PA increased by 23.25% at 1 h after batroxobin administration in a dose of 1.0 BU/kg as compared with the control group. Fbg could be remarkably decreased after batroxobin administration in a dose of 0.5, 1.0 BU/kg, which was prominent after 6 h of administration. Batroxobin had no remarkable effect on the incidence and types of cerebral hemorrhage. Conclusion The method of rabbit embolic stroke model by injecting micro Jolood clots into MCA is successful and may be reliable in evaluation of thrombolytic drugs.